Recent Publications

Efficient Generation of Genome-wide Libraries for Protein–ligand Screens Using Gibson Assembly.

Sternlieb, T., Loock, M., Gao, M. and Cestari, I.

Bio-protocol 2022, 12(22): e4558. DOI: 10.21769/BioProtoc.4558.

Genome-wide libraries for protozoan pathogens drug target screening using yeast surface display

Heslop R, Gao M, Brito-Lira A, Sternlieb T, Loock M, Sanghi SR, Cestari I

bioRxiv, 2022 Nov 17. doi: https://doi.org/10.1101/2022.11.17.516844

The current drug discovery landscape for trypanosomiasis and leishmaniasis: Challenges and strategies to identify drug targets.

Altamura F, Rajesh R, Catta-Preta CMC, Moretti NS, Cestari I.

Drug Dev Res. 2022 Apr;83(2):225-252. doi: 10.1002/ddr.21664. Epub 2020 Apr 6. PMID: 32249457 

Phosphoinositide signaling and regulation in Trypanosoma brucei: Specialized functions in a protozoan pathogen.

Cestari I.

PLoS Pathog. 2020 Jan 2;16(1):e1008167. doi: 10.1371/journal.ppat.1008167. eCollection 2020 Jan.PMID: 31895930 

Nuclear Phosphatidylinositol 5-Phosphatase Is Essential for Allelic Exclusion of Variant Surface Glycoprotein Genes in Trypanosomes.

Cestari I, McLeland-Wieser H, Stuart K. 

Mol Cell Biol. 2019 Jan 16;39(3):e00395-18. doi: 10.1128/MCB.00395-18. PMID: 30420356; PMCID: PMC6336139. Selected postdoctoral work.

Chemogenetic Characterization of Inositol Phosphate Metabolic Pathway Reveals Druggable Enzymes for Targeting Kinetoplastid Parasites.

Cestari I, Haas P, Moretti NS, Schenkman S, Stuart K. 

Cell Chem Biol. 2016 May 19;23(5):608-617. doi: 10.1016/j.chembiol.2016.03.015. Epub 2016 Apr 28.PMID: 27133314. Selected postdoctoral work. 

Inositol phosphate pathway controls transcription of telomeric expression sites in trypanosomes. 

Cestari I, Stuart K.

Proc Natl Acad Sci U S A. 2015 May 26;112(21):E2803-12. doi: 10.1073/pnas.1501206112. Epub 2015 May 11. PMID: 25964327; PMCID: PMC4450425. Selected postdoctoral work.

 

Publications with collaborators

Protein acetylation in the critical biological processes in protozoan parasites

Maran SR, Fleck K, Monteiro-Teles NM, Isebe T, Walrad P, Jeffers V, Cestari I, Vasconcelos EJR, Moretti N. 

Trends Parasitol. 2021 May 12;S1471-4922(21)00088-X.  doi: 10.1016/j.pt.2021.04.008.

ATR Kinase Is a Crucial Player Mediating the DNA Damage Response in Trypanosoma brucei.

Marin PA, Obonaga R, Pavani RS, da Silva MS, de Araujo CB, Lima AA, Avila CC, Cestari I, Machado CR, Elias MC.

Front Cell Dev Biol. 2020 Dec 22;8:602956. doi: 10.3389/fcell.2020.602956. eCollection 2020.PMID: 33415107 

Lexis and Grammar of Mitochondrial RNA Processing in Trypanosomes.

Aphasizheva I, Alfonzo J, Carnes J, Cestari I, Cruz-Reyes J, Göringer HU, Hajduk S, Lukeš J, Madison-Antenucci S, Maslov DA, McDermott SM, Ochsenreiter T, Read LK, Salavati R, Schnaufer A, Schneider A, Simpson L, Stuart K, Yurchenko V, Zhou ZH, Zíková A, Zhang L, Zimmer S, Aphasizhev R.

Trends Parasitol. 2020 Apr;36(4):337-355. doi: 10.1016/j.pt.2020.01.006. Epub 2020 Feb 28.PMID: 32191849 

The phosphoinositide regulatory network in Trypanosoma brucei: Implications for cell-wide regulation in eukaryotes.

Cestari I, Stuart K.

PLoS Negl Trop Dis. 2020 Oct 29;14(10):e0008689. doi: 10.1371/journal.pntd.0008689. eCollection 2020 Oct.PMID: 33119588 

Plasmodium Secretion Induces Hepatocyte Lysosome Exocytosis and Promotes Parasite Entry.

Vijayan K, Cestari I, Mast FD, Glennon EKK, McDermott SM, Kain HS, Brokaw AM, Aitchison JD, Stuart K, Kaushansky A.

iScience. 2019 Nov 22;21:603-611. doi: 10.1016/j.isci.2019.10.054. Epub 2019 Oct 31.PMID: 31731198